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KMID : 0545119990090010032
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Journal of Microbiology and Biotechnology
1999 Volume.9 No. 1 p.32 ~ p.38
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Purification and Characterixation of Biosurfactant from Tsukamurella sp.26A
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Choi, Kyung Suk
Kim, Soon Han/Lee, Tae Ho
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Abstract
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A biosurfactant produced by Tsukamurella sp. 26A was purified by procedures including acid precipitation, ethylacetate extraction. and adsorption chromatography. The purified biosurfactant reduced the surface tension of water from 72 mN/m to 30 mN/m at a concentration of 250 ㎎/l, whereas the minimum interfacial tension against n-hexadecane was lowered to 1.5 mN/m at a concentration of 40 ㎎/l. The compound stabilized oil-in-water emulsions with a variety of commercial oils and had strong emulsification and stabilization activities when compared to those of commercial emulsifiers and stabilizers. Surface tension was stable over a broad range of pH (2∼12) and temperature (100℃. 3h). The biosurfactant was identified as glycolipid having a hydrophilic moiety of trehalose.
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